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Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus BJM
Flatschart,Roberto B.; Resende,Maurício.
Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aujeszky’s disease virus; Polymerase chain reaction; Dot-blot hybridization.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013
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Antibodies against pseudorabies virus in feral swine in southeast Brazil Arq. Bras. Med. Vet. Zootec.
Cunha,E.M.S.; Nassar,A.F.C.; Lara,M.C.C.S.H.; Bersano,J.G.; Villalobos,E.M.C.; Oliveira,J.C.F..
Serum samples collected from 358 wild boars (Sus scrofa) in breeding farms in São Paulo, southeast Brazil, from 1998 to 2001, were tested for antibodies against pseudorabies virus (PRV) by means of serum neutralization (SN) and enzyme-linked immunobsorbent assay (ELISA). Seropositive animals were detected in three of seven herds analyzed. Overall seroprevalence as assessed by SN was 30.7%, ranging from 25.2% to 100% for the herds that presented seropositive animals. Indirect ELISA detected lower seroprevalence (19.3%). Sensitivity and specificity of ELISA were equal to 57.3% and 97.6%, respectively. Agreement was equal to 85.2% (P<0.0001). These results showed that PRV infections occurred in farmed feral swine in southeast Brazil, and affect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wild boar; Sus scrofa; Pseudorabies virus; Aujeszky’s disease virus; Antibodies.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400002
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Partial characterization of a Brazilian strain of Aujeszky’s disease virus recovered from a pig with subclinical infection Arq. Bras. Med. Vet. Zootec.
Cândido,A.L.; Resende,M.; Oliveira,M.E.; Bontempo,E.D.; Drumond,B. P..
One Brazilian strain of Aujeszky’s disease virus isolated from a piglet in which the disease had not been observed was studied as for its virulence in pigs. The genome of the virus was molecularly analysed as for their restriction endonuclease cleavage pattern. Fifty-day-old non-immune weanlings exposed to this strain showed no disease although the virus was present in their oropharyngeal area for at least three days. All animals developed moderate titers of neutralizing antibody. Based on number of bands and migration rate of restriction fragments the isolate was classified into Herrmann’s type I group. Latent infection was detected in all pigs by PCR. Some variations were detected in the cleavage pattern of the strain ASB Piau when compared to LA031...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Swine; Aujeszky’s disease virus; Virulence; Latency.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400001
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